Novel on-line sample preconcentration technique in microchip micellar electrokinetic chromatography: Development of transient-trapping.

Development of a Novel On-line Sample Preconcentration and Separation Technique in Microchip Micellar Electrokinetic Chromatography

Fluorescence Imaging Analysis of Transient Trapping–microchip Micellar Electrokinetic Chromatography

This paper reports an fluorescence imaging analysis to verify the proposed mechanisms in transient trapping– microchip micellar electrokinetic chromatography (tr-trapping–MCMEKC). Fluorescence imaging revealed that a process of tr-trapping consisted of four migration stages with different apparent diffusion constants. In the first trapping stage, the diffusion of a focused sample band was suppr...

Metal complex separation with on-line sample preconcentration in micellar electrokinetic chromatography.

Micellar electrokinetic chromatography (MEKC) using a cationic surfactant as a pseudostationary phase was examined to separate anionic metal cyclohexane-1,2-diaminetetraacetic acid (CDTA) complexes. Cetyltrimethylammonium chloride (CTAC) was employed as the cationic surfactant micelle, its addition leading to EOF reversal. Cu(II), Co(II), Zn(II), Mn(II) and Pb(II) were used as test analytes, an...

Effect of a low-conductivity zone on field-amplified sample stacking in microchip micellar electrokinetic chromatography.

The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume inj...

On-line preconcentration and determination of ketamine and norketamine by micellar electrokinetic chromatography. Complementary method to gas chromatography/mass spectrometry.

We have investigated a rapid, simple, and highly efficient on-line preconcentration method using in micellar electrokinetic chromatography (MEKC) for the analysis of abused drugs. Ketamine is an anesthetic that has been abused as a hallucinogen. We applied the sample sweeping technique first to ketamine and its major metabolite, norketamine, and separated the analytes with MEKC. Several of the ...